citrate buffer (Thermo Fisher)

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Thermo Fisher citrate buffer
Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/assay+buffer/Citrate+Buffer/10__22203_slash_ecm__v033a11-128-23-28
Average 99 stars, based on 1 article reviews

citrate buffer - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: Identification of potent and selective small molecule TALK-1 inhibitors that promote β-cell function.
Article Snippet: The two-pore domain K (K2P) channel TALK-1 is a key regulator of ꞵ-cell electrical excitability, Ca handling, and glucose-stimulated insulin secretion (GSIS).. KCNK16, the gene encoding TALK-1, is the most abundant ꞵ-cell K channel transcript and the most islet-restricted ion channel.. Genetic evidence also links KCNK16 to diabetic phenotypes, including a gain-of-function (GOF) polymorphism (rs1535500) that predisposes individuals to type 2 diabetes (T2D) and a GOF mutation (p.L114P) that causes maturity-onset diabetes of the young.

Article Title: Spiromacrocyclic orexin 2 receptor agonists
Article Snippet: .. The following day, the media was removed, and the cells were incubated with assay buffer (HBSS with 20 mM HEPES, pH 7.4) containing 2.5 mM probenecid (Thermo Fisher Scientific), 10 mM CaCl2, 0.1% pluronic F68 (Gibco) and 1× calcium-4 dye (Molecular Devices) for 1 h at 37° C. and 5% CO2. .. After incubation, cells were allowed to equilibrate at room temperature for 15 minutes and then loaded into FDSS7000Ex system (Hamamatsu Photonics) together with a plate containing test compounds serially diluted in assay buffer.

Article Title: Transient Increase in AT 1 R Expression at the Myocardial Infarct Site Is Associated with Early Fibrotic Remodeling in Infarcted Rat Heart.
Article Snippet: .. Briefly, on the day of the experiment, slides were incubated at RT for 15 min in assay buffer (150 mM NaCl (Thermo Fischer Scientific, Waltham, MA, USA), 50 mM Na2HPO4 dibasic (VWR, Radnor, PA, USA), 1 mM EDTA (Wisent Inc., Saint-Jean-Baptiste, QC, Canada), 0.1 mM bacitracin (Thermo Fischer Scientific, Waltham, MA, USA) and 0.1% BSA (pH 7.4, VWR, Radnor, PA, USA). .. Slides were then incubated with 0.25 nM of 125I-Sar1-Ile8-Ang II (Perkin Elmer, Shelton, CT, USA) for 90 min at RT along with AT2R antagonist PD 123, 319 (10 μM, Alomone Labs, Jerusalem, Israel) to obtain total non-AT2R binding (TB), or with unlabeled Ang II (10 μM, Alomone Labs, Jerusalem, Israel) to determine non-specific binding (NSB).

Article Title: The double-edged nature of antibody bivalency: mathematical and experimental analysis of cell surface antigen occupancy and opsonization.
Article Snippet: Sensorgrams were fitted to a 1:1 binding model with Rmax local, from which kinetic rate constants (kon and koff) and affinity (KD) was determined using the Biacore Insight Evaluation software v5 (Cytiva). .. Titrations of antibodies (2.09 × 10−13 − 1 × 10−6 M), prepared in an assay buffer (PBS (Gibco 14,190,144) supplemented with 0.5% v/v bovine serum albumin (Sigma Aldrich A7979) and 2 mM EDTA (Sigma Aldrich E7889), were incubated with recombinant CHO cell lines possessing varying densities of human PD-1 for one hour at 25°C. .. Supernatant was then aspirated, and cells were washed with assay buffer twice, and APC-conjugated goat antihuman IgG (1:50 in assay buffer, Jackson ImmunoResearch 109–136-127) was added to all samples for one hour at 25°C.

Concentration Assay:

Article Title: Ligand binding modes of the bitter taste receptor T2R14 and T2R46.
Article Snippet: .. Various ligands, including strychnine (Qchen Bio), tangeretin (Sigma Aldrich), artemisinin (Selleck), denatonium benzoate (Sigma Aldrich) and chlorhexidine (Sigma Aldrich), were dissolved in dimethyl sulfoxide (DMSO) or deionized water (ddH20) at a stock concentration of 50 mM and diluted subsequently to working concentrations ranging from 64 nM to 5 mM using assay buffer (HBSS buffer from Thermo Fisher Scientific, supplemented with 20 mM HEPES, pH 7.4). ..

Article Title: Ligand binding modes of the bitter taste receptor T2R14 and T2R46
Article Snippet: .. Various ligands, including strychnine (Qchen Bio), tangeretin (Sigma Aldrich), artemisinin (Selleck), denatonium benzoate (Sigma Aldrich) and chlorhexidine (Sigma Aldrich), were dissolved in dimethyl sulfoxide (DMSO) or deionized water (ddH 2 0) at a stock concentration of 50 mM and diluted subsequently to working concentrations ranging from 64 nM to 5 mM using assay buffer (HBSS buffer from Thermo Fisher Scientific, supplemented with 20 mM HEPES, pH 7.4). ..

Recombinant:

Saline:

Article Title: Confirming ERVEBO Vaccination to Support Ebola Virus Surveillance
Article Snippet: We bound the biotinylated Avi-tagged EBOV GP1,2, EBOV sGP, and VSV-P-N to avidin-coupled magnetic microspheres (Diasorin), following manufacturer instructions. .. We removed the storage buffer by magnetic separation and then resuspended the beads in assay buffer (phosphate-buffered saline [PBS; ThermoFisher Scientific] with 1% bovine serum albumin [BSA; Millipore Sigma, https://www.sigmaaldrich.com ]). ..

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